当前位置：首页>郑州>郑州大学张书胜&邹丽娜‖【AC】巧用“位点占据”:正交DNA探针与磷酸盐效应协同的新型全分裂式光电传感平台实现多重精准检测

郑州大学张书胜&邹丽娜‖【AC】巧用“位点占据”:正交DNA探针与磷酸盐效应协同的新型全分裂式光电传感平台实现多重精准检测

  • 2026-03-31 10:36:31
郑州大学张书胜&邹丽娜‖【AC】巧用“位点占据”:正交DNA探针与磷酸盐效应协同的新型全分裂式光电传感平台实现多重精准检测

点击蓝字

关注我们

介绍 /Introduction

为了克服传统光电化学适体传感器的局限性——例如电极修饰复杂、界面干扰以及多重检测能力受限——本研究提出了一种基于DNA分子逻辑门和磷酸盐驱动释放机制的"全分裂式"光电化学传感策略。可编程DNA逻辑门完全在均相溶液中运行，无需外部调控即可实现多种靶标的同时识别和信号探针的释放。磷酸根离子进一步将DNA探针从锆基金属有机框架信号标签（UiO-66-TCPP）上竞争性置换下来。释放的MOFs被直接滴涂到裸电极上，产生无背景干扰的光电化学信号。通过将所有识别步骤移至溶液中并免去电极修饰需求，该方法最大限度地减少了界面相关问题，并将检测简化为单一的滴涂步骤。由此构建的平台在保持高选择性和内在可编程性的同时，提升了简便性、稳定性和检测效率。此外，这种通用的裸电极读出模式易于与小型化系统兼容，为实现多重化和便携式光电化学生物分析提供了一条通用且实用的途径。
相关成果以 “ A Fully Split-Type PEC Sensing Driven by Orthogonal DNA Nanoprobes and Phosphate-Induced Site-Occupation Effect for Multiplexed Detection” 为题发表在《Anal. Chem. 》。
「全文速递」郑州大学张书胜&邹丽娜‖AC巧用位点占据：正交DNA探针与磷酸盐效应协同的新型全分裂式光电传感平台实现多重精准检测.pdf

研究图文/Introduction

Scheme 1. Principle of the Fully Split-Type PEC Aptasensor

Figure 1. Morphology and structure characteristics. TEM images of (A) UiO-66, (B) UiO-66-TCPP, and (C) MP-UiO-66-TCPP and (D) EDS of it. The high-resolution XPS spectra of (E) Zr 3d, (F) O 1s, and (G) P 2p for MP-UiO-66-TCPP. (H) XRD pattern of as-prepared samples.

Figure 2. (A) Photocurrent responses of different electrodes in Tris-HCl (0.1 M, pH 7.4) containing 0.1 M AA, (B) photocurrent responses with different targets in Tris-HCl (0.1 M, pH 7.4) containing 0.1 M AA (target concentration: 100 pM), (C) PAGE characterization of the target recognition process, and (D) fluorescence characterization of the site-occupying effect.

Figure 3. Work efficiency of the two different sensors: CPEC and NPEC (target concentration: 10 nM).
Figure 4. Optimization of concentration of the PO43- (A,C) and reaction time of the PO43- (B,D).
Figure 5. (A) Photocurrent responses of different AMOX concentrations (from a to h: 10 fM, 100fM, 1pM, 10 pM, 100 pM, 1 nM, and 10 nM) and (B) corresponding calibration curve (error bar: SD; n = 3). (C) Photocurrent responses of different ACE concentrations (from a to h: 10 fM, 100 fM, 1 pM, 10 pM, 100 pM, 1 nM, and 10 nM) and (D) corresponding calibration curve (error bar: SD; n = 3). The selectivity of the photoelectrochemical biosensor for (E) AMOX detection and (F) ACE detection (The concentration of interferents was 10 nM; targets were prepared at a concentration of 100pM.). (G) The stability and (H) reproducibility of the photoelectrochemical biosensor (targets concentration: 100 pM).
Figure 6. Illustration of the combinatorial DNA molecular logic operation.

结论 /Conclusion

本研究提出了一种创新的全分离式光电化学适配体传感器，用于环境样本中快速、超灵敏且无背景干扰的多靶点检测。该系统将目标识别、信号分子解离和光电化学读出模块化为三个独立单元：DNA逻辑门实现了特定且可编程的多靶点识别，磷酸盐诱导的金属有机框架解离充当通用的光电电子标签，裸电极则作为通用的信号读出平台。通过将检测的“智能”核心完全限制在均相中运行，传感过程避开了复杂的电极功能化步骤。这种设计不仅简化了传感器的制造，缩短了检测时间，还解决了固定探针系统中常见的空间位阻、生物分子失活和信号串扰等问题。值得注意的是，该平台能够独立识别和同时检测多靶点，无需针对特定目标的输出模块、定制的识别界面或外部参数调整。其在便携式工作站上的成功运行证实了其实用性，为新型生物测定设计提供了框架，并有助于开发用于现场环境监测、农业污染物筛查和生物医学废水分析的便携式设备。

文献直通车 /Literature information

A Fully Split-Type PEC Sensing Driven by Orthogonal DNA Nanoprobes and Phosphate-Induced Site-Occupation Effect for Multiplexed DetectionJiaxin Guo, Shuting Wang, Gaiping Li, Baoxian Ye, Shusheng Zhang,* and Lina Zou*原文链接：https://doi.org/10.1021/acs.analchem.6c00213

点击左下角“阅读原文”，轻松获取原文信息

扫描上侧二维码

关注我们

END

投稿、荐稿、合作请联系邮箱

food_sensing_lab@163.com

本文来自网友投稿或网络内容，如有侵犯您的权益请联系我们删除，联系邮箱：wyl860211@qq.com 。

高价政策,初七结束,郑州富士康盛大开启普工招募,返费工、小时工同步招聘

郑州市这2县城有望联手合并,如成功,或将有望晋升县级市

最新文章

随机文章

基本 文件 流程 错误 SQL 调试
  1. 请求信息 : 2026-04-25 11:13:49 HTTP/2.0 GET : https://i.460.net.cn/a/482569.html
  2. 运行时间 : 0.086529s [ 吞吐率：11.56req/s ] 内存消耗：4,416.64kb 文件加载：140
  3. 缓存信息 : 0 reads,0 writes
  4. 会话信息 : SESSION_ID=2e55aa31cba058fac610aa5975c2f062
  1. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/public/index.php ( 0.79 KB )
  2. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/autoload.php ( 0.17 KB )
  3. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/composer/autoload_real.php ( 2.49 KB )
  4. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/composer/platform_check.php ( 0.90 KB )
  5. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/composer/ClassLoader.php ( 14.03 KB )
  6. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/composer/autoload_static.php ( 4.90 KB )
  7. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-helper/src/helper.php ( 8.34 KB )
  8. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-validate/src/helper.php ( 2.19 KB )
  9. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/helper.php ( 1.47 KB )
  10. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/stubs/load_stubs.php ( 0.16 KB )
  11. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Exception.php ( 1.69 KB )
  12. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-container/src/Facade.php ( 2.71 KB )
  13. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/symfony/deprecation-contracts/function.php ( 0.99 KB )
  14. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/symfony/polyfill-mbstring/bootstrap.php ( 8.26 KB )
  15. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/symfony/polyfill-mbstring/bootstrap80.php ( 9.78 KB )
  16. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/symfony/var-dumper/Resources/functions/dump.php ( 1.49 KB )
  17. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-dumper/src/helper.php ( 0.18 KB )
  18. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/symfony/var-dumper/VarDumper.php ( 4.30 KB )
  19. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/App.php ( 15.30 KB )
  20. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-container/src/Container.php ( 15.76 KB )
  21. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/psr/container/src/ContainerInterface.php ( 1.02 KB )
  22. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/app/provider.php ( 0.19 KB )
  23. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Http.php ( 6.04 KB )
  24. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-helper/src/helper/Str.php ( 7.29 KB )
  25. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Env.php ( 4.68 KB )
  26. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/app/common.php ( 0.03 KB )
  27. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/helper.php ( 18.78 KB )
  28. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Config.php ( 5.54 KB )
  29. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/config/app.php ( 0.95 KB )
  30. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/config/cache.php ( 0.78 KB )
  31. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/config/console.php ( 0.23 KB )
  32. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/config/cookie.php ( 0.56 KB )
  33. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/config/database.php ( 2.47 KB )
  34. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/facade/Env.php ( 1.67 KB )
  35. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/config/filesystem.php ( 0.61 KB )
  36. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/config/lang.php ( 0.91 KB )
  37. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/config/log.php ( 1.35 KB )
  38. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/config/middleware.php ( 0.19 KB )
  39. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/config/route.php ( 1.89 KB )
  40. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/config/session.php ( 0.57 KB )
  41. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/config/trace.php ( 0.34 KB )
  42. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/config/view.php ( 0.82 KB )
  43. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/app/event.php ( 0.25 KB )
  44. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Event.php ( 7.67 KB )
  45. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/app/service.php ( 0.13 KB )
  46. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/app/AppService.php ( 0.26 KB )
  47. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Service.php ( 1.64 KB )
  48. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Lang.php ( 7.35 KB )
  49. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/lang/zh-cn.php ( 13.70 KB )
  50. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/initializer/Error.php ( 3.31 KB )
  51. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/initializer/RegisterService.php ( 1.33 KB )
  52. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/services.php ( 0.14 KB )
  53. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/service/PaginatorService.php ( 1.52 KB )
  54. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/service/ValidateService.php ( 0.99 KB )
  55. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/service/ModelService.php ( 2.04 KB )
  56. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-trace/src/Service.php ( 0.77 KB )
  57. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Middleware.php ( 6.72 KB )
  58. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/initializer/BootService.php ( 0.77 KB )
  59. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/Paginator.php ( 11.86 KB )
  60. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-validate/src/Validate.php ( 63.20 KB )
  61. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/Model.php ( 23.55 KB )
  62. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/model/concern/Attribute.php ( 21.05 KB )
  63. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/model/concern/AutoWriteData.php ( 4.21 KB )
  64. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/model/concern/Conversion.php ( 6.44 KB )
  65. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/model/concern/DbConnect.php ( 5.16 KB )
  66. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/model/concern/ModelEvent.php ( 2.33 KB )
  67. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/model/concern/RelationShip.php ( 28.29 KB )
  68. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-helper/src/contract/Arrayable.php ( 0.09 KB )
  69. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-helper/src/contract/Jsonable.php ( 0.13 KB )
  70. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/model/contract/Modelable.php ( 0.09 KB )
  71. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Db.php ( 2.88 KB )
  72. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/DbManager.php ( 8.52 KB )
  73. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Log.php ( 6.28 KB )
  74. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Manager.php ( 3.92 KB )
  75. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/psr/log/src/LoggerTrait.php ( 2.69 KB )
  76. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/psr/log/src/LoggerInterface.php ( 2.71 KB )
  77. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Cache.php ( 4.92 KB )
  78. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/psr/simple-cache/src/CacheInterface.php ( 4.71 KB )
  79. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-helper/src/helper/Arr.php ( 16.63 KB )
  80. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/cache/driver/File.php ( 7.84 KB )
  81. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/cache/Driver.php ( 9.03 KB )
  82. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/contract/CacheHandlerInterface.php ( 1.99 KB )
  83. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/app/Request.php ( 0.09 KB )
  84. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Request.php ( 55.78 KB )
  85. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/app/middleware.php ( 0.25 KB )
  86. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Pipeline.php ( 2.61 KB )
  87. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-trace/src/TraceDebug.php ( 3.40 KB )
  88. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/middleware/SessionInit.php ( 1.94 KB )
  89. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Session.php ( 1.80 KB )
  90. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/session/driver/File.php ( 6.27 KB )
  91. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/contract/SessionHandlerInterface.php ( 0.87 KB )
  92. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/session/Store.php ( 7.12 KB )
  93. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Route.php ( 23.73 KB )
  94. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/route/RuleName.php ( 5.75 KB )
  95. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/route/Domain.php ( 2.53 KB )
  96. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/route/RuleGroup.php ( 22.43 KB )
  97. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/route/Rule.php ( 26.95 KB )
  98. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/route/RuleItem.php ( 9.78 KB )
  99. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/route/app.php ( 1.72 KB )
  100. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  101. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/route/dispatch/Controller.php ( 4.74 KB )
  102. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/route/Dispatch.php ( 10.44 KB )
  103. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/app/controller/Index.php ( 4.81 KB )
  104. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/app/BaseController.php ( 2.05 KB )
  105. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/facade/Db.php ( 0.93 KB )
  106. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/connector/Mysql.php ( 5.44 KB )
  107. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/PDOConnection.php ( 52.47 KB )
  108. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/Connection.php ( 8.39 KB )
  109. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/ConnectionInterface.php ( 4.57 KB )
  110. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/builder/Mysql.php ( 16.58 KB )
  111. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/Builder.php ( 24.06 KB )
  112. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/BaseBuilder.php ( 27.50 KB )
  113. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/Query.php ( 15.71 KB )
  114. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/BaseQuery.php ( 45.13 KB )
  115. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/concern/TimeFieldQuery.php ( 7.43 KB )
  116. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/concern/AggregateQuery.php ( 3.26 KB )
  117. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/concern/ModelRelationQuery.php ( 20.07 KB )
  118. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/concern/ParamsBind.php ( 3.66 KB )
  119. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/concern/ResultOperation.php ( 7.01 KB )
  120. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/concern/WhereQuery.php ( 19.37 KB )
  121. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/concern/JoinAndViewQuery.php ( 7.11 KB )
  122. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/concern/TableFieldInfo.php ( 2.63 KB )
  123. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-orm/src/db/concern/Transaction.php ( 2.77 KB )
  124. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/log/driver/File.php ( 5.96 KB )
  125. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/contract/LogHandlerInterface.php ( 0.86 KB )
  126. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/log/Channel.php ( 3.89 KB )
  127. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/event/LogRecord.php ( 1.02 KB )
  128. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-helper/src/Collection.php ( 16.47 KB )
  129. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/facade/View.php ( 1.70 KB )
  130. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/View.php ( 4.39 KB )
  131. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
  132. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/response/View.php ( 3.29 KB )
  133. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/Cookie.php ( 6.06 KB )
  134. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-view/src/Think.php ( 8.38 KB )
  135. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/framework/src/think/contract/TemplateHandlerInterface.php ( 1.60 KB )
  136. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-template/src/Template.php ( 46.61 KB )
  137. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-template/src/template/driver/File.php ( 2.41 KB )
  138. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-template/src/template/contract/DriverInterface.php ( 0.86 KB )
  139. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/runtime/temp/ea612fc1da910b74827694dd9af81119.php ( 11.98 KB )
  140. /yingpanguazai/ssd/ssd1/www/i.460.net.cn/vendor/topthink/think-trace/src/Html.php ( 4.42 KB )
  1. CONNECT:[ UseTime:0.000422s ] mysql:host=127.0.0.1;port=3306;dbname=i460;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `fenlei` [ RunTime:0.000548s ]
  3. SELECT * FROM `fenlei` WHERE `fid` = 0 [ RunTime:0.004140s ]
  4. SELECT * FROM `fenlei` WHERE `fid` = 63 [ RunTime:0.000316s ]
  5. SHOW FULL COLUMNS FROM `set` [ RunTime:0.000505s ]
  6. SELECT * FROM `set` [ RunTime:0.000413s ]
  7. SHOW FULL COLUMNS FROM `article` [ RunTime:0.000632s ]
  8. SELECT * FROM `article` WHERE `id` = 482569 LIMIT 1 [ RunTime:0.000785s ]
  9. UPDATE `article` SET `lasttime` = 1777086829 WHERE `id` = 482569 [ RunTime:0.000578s ]
  10. SELECT * FROM `fenlei` WHERE `id` = 64 LIMIT 1 [ RunTime:0.000256s ]
  11. SELECT * FROM `article` WHERE `id` < 482569 ORDER BY `id` DESC LIMIT 1 [ RunTime:0.002219s ]
  12. SELECT * FROM `article` WHERE `id` > 482569 ORDER BY `id` ASC LIMIT 1 [ RunTime:0.000499s ]
  13. SELECT * FROM `article` WHERE `id` < 482569 ORDER BY `id` DESC LIMIT 10 [ RunTime:0.000659s ]
  14. SELECT * FROM `article` WHERE `id` < 482569 ORDER BY `id` DESC LIMIT 10,10 [ RunTime:0.000740s ]
  15. SELECT * FROM `article` WHERE `id` < 482569 ORDER BY `id` DESC LIMIT 20,10 [ RunTime:0.000724s ]
0.088084s