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国产一区期刊FQS|文章导读|郑州轻工业大学白艳红团队在牛肉基质干扰免疫磁珠分离大肠杆菌O157:H7及机制研究方面取得进展

  • 2026-06-08 11:28:36
国产一区期刊FQS|文章导读|郑州轻工业大学白艳红团队在牛肉基质干扰免疫磁珠分离大肠杆菌O157:H7及机制研究方面取得进展
Food Quality and Safety
IF=4.4，农林科学一区
国产期刊 (CN号: 33-1407/TS)

大肠杆菌O157:H7（Escherichia coli O157:H7）是牛肉及其制品中典型的高危食源性致病菌，可引起出血性腹泻、出血性结肠炎等严重病症，甚至导致死亡。免疫磁珠分离技术能够特异性识别并快速分离靶细菌，是食源性致病菌高效富集的重要前处理手段，可显著提高检测灵敏度与准确性、缩短检测时间。但现有研究表明，食品基质成分会对磁分离效率产生明显干扰，复杂牛肉基质尤甚，可大幅降低免疫磁珠的捕获效率。目前其干扰规律与内在机制仍不明确，严重制约了该技术在肉品安全检测中的应用。

近日，郑州轻工业大学白艳红团队在Food Quality and Safety(FQS)发表了题为"Interference of beef matrix on the magnetic separation of Escherichia coli O157:H7 by immunomagnetic beads and the underlying mechanism"的研究论文（文章链接https://doi.org/10.1093/fqsafe/fyag009，全文免费下载牛肉基质对免疫磁珠磁分离大肠杆菌O157:H7的干扰作用及其机制研究.pdf)。

本研究以大肠杆菌O157:H7为研究对象，构建了牛肉中大肠杆菌O157:H7免疫磁珠高效分离体系，揭示了牛肉基质对磁分离效率的影响规律及干扰机制。

主要结论如下：（1）牛肉基质可显著降低免疫磁珠对大肠杆菌O157:H7的捕获效率，牛肉蛋白是关键干扰因子，其中肌浆蛋白与肌原纤维蛋白浓度均与捕获率呈显著负相关；（2）明确了牛肉蛋白通过改变免疫磁珠表面性质、降低抗体负载量、削弱靶标结合亲和性等途径干扰磁分离效率；（3）从蛋白二级结构、溶解性、表面疏水性及分子间相互作用等层面，揭示了牛肉基质的干扰机制。

图1. 牛肉基质及其主要蛋白对免疫磁珠捕获效率的影响

图2. 牛肉肌浆蛋白（SP）与肌原纤维蛋白（MP）对免疫磁珠（IMBs）微观结构的干扰作用

研究结果阐明了牛肉基质关键干扰成分对免疫磁珠识别分离靶细菌的干扰机制，可为针对不同食品建立抗基质干扰的高效磁分离方法提供理论依据，为食源性致病菌快速、精准、高灵敏检测提供技术支撑。

本研究得到了国家自然科学基金（32402241）项目的资助。

作者介绍

通讯作者

 通讯作者

第一作者

杜娟，郑州轻工业大学食品与生物工程学院副教授、硕士生导师，主要从事食品微生物快检技术科研教学工作，主持国家自然科学基金、河南省科技攻关等项目，作为主要完成人获河南省科学技术进步奖一等奖。

通讯作者

白艳红，博士，二级教授，博士生导师，郑州轻工业大学冷链食品加工与安全控制教育部重点实验室（培育）主任，河南工业大学副校长，国务院政府特殊津贴专家、中原科技创新领军人才、河南省学术技术带头人。长期致力于肉品加工与安全控制、食品安全检测与防控、食品非热加工新技术等方面的研究工作。主持国家自然科学基金面上项目2项、“十三五”国家重点研发计划子课题、河南省重大科技专项、重大公益专项等10余项，作为第一完成人获河南省科学技术进步奖一等奖、二等奖各1项、中国轻工业联合会科技进步奖一等奖、二等奖各1项，授权发明专利14件，第一或通讯作者发表SCI/EI收录论文100余篇（其中TOP期刊40篇，ESI高被引论文4篇）。

https://doi.org/10.1093/fqsafe/fyag018

（开放获取，全文可免费阅读、下载牛肉基质对免疫磁珠磁分离大肠杆菌O157:H7的干扰作用及其机制研究.pdf)

2026年代表性论文鉴赏(2)

Quality evaluation of NMN produced via different synthesis methods: A multi-technique analysis using NMR, IR, GC-TCD, and HPLC-UV

不同合成工艺制备 β- 烟酰胺单核苷酸（NMN）的品质评价：基于核磁共振、红外光谱、气相色谱-热导检测与紫外高效液相色谱联用的多技术分析

原文链接：http://dx.doi.org/10.1093/fqsafe/fyaf067    

PDF下载：fyaf067.pdf 

Valorizing animal waste protein through spinning: sustainable nanofiber innovation for advanced food packaging

利用纺丝技术实现畜禽废弃蛋白资源化：面向高端食品包装的可持续纳米纤维研发

原文链接：http://dx.doi.org/10.1093/fqsafe/fyaf042    

PDF下载：fyaf042.pdf 

Strigolactone analog 7FGR24 enhances storability of fresh Gastrodia elata by modulating plant hormone signaling

独脚金内酯类似物7FGR24通过调控植物激素信号通路提升新鲜天麻贮藏性能

原文链接：http://dx.doi.org/10.1093/fqsafe/fyaf069    

PDF下载：fyaf069.pdf 

Interference of beef matrix in the magnetic separation of Escherichia coli O157:H7 by immunomagnetic beads and the underlying mechanism

牛肉基质对免疫磁珠分选大肠杆菌 O157:H7 的干扰作用及其内在机理

原文链接：http://dx.doi.org/10.1093/fqsafe/fyag009

PDF下载：fyag009.pdf 

Melatonin treatment alleviates chilling injury and maintains quality attributes in cold-stored Chinese olives via modulating energy metabolism

褪黑素处理通过调控能量代谢减轻冷藏青榄冷害并维持其品质指标

原文链接：http://dx.doi.org/10.1093/fqsafe/fyag013    

PDF下载：fyag013.pdf 

Mechanism of reduced steamed bun hardness via the addition of single helical corn amylopectin

添加单螺旋结构玉米支链淀粉降低馒头硬度的作用机理

原文链接：http://dx.doi.org/10.1093/fqsafe/fyag002  

PDF下载：fyag002.pdf 

Synergistic effects of co-delivery bioactive compounds using polymeric-based nanoparticles for biological activities: recent trends

高分子基纳米颗粒共递送生物活性成分的生物活性协同作用：前沿研究进展

原文链接：http://dx.doi.org/10.1093/fqsafe/fyaf061  

PDF下载：fyaf061.pdf 

Effects of medium-chain triglyceride ketogenic diets on glucose metabolism in type 2 diabetic mice

中链甘油三酯生酮饮食对2型糖尿病小鼠糖代谢的影响

原文链接：http://dx.doi.org/10.1093/fqsafe/fyag011  

PDF下载：fyag011.pdf 

The mechanism underlying deoxynivalenol-induced damage and prospective nutritional regulation strategies

脱氧雪腐镰刀菌烯醇致损伤的作用机理及潜在营养调控策略

原文链接：http://dx.doi.org/10.1093/fqsafe/fyaf059

PDF下载：fyaf059.pdf 

Exogenous methyl jasmonate alleviates chilling injury in postharvest green-peel ripening mango cv. 'Guire 82' by boosting the antioxidative system and affecting energy metabolism

外源茉莉酸甲酯通过激活抗氧化系统、调控能量代谢缓解采后桂热 82 号青皮成熟芒果的冷害

原文链接：http://dx.doi.org/10.1093/fqsafe/fyaf066    

PDF下载：fyaf066.pdf 

关于期刊

Food Quality and Safety（FQS）期刊由浙江大学主办，浙江大学出版社与牛津大学出版社合作出版，是一本国产英文期刊。由浙江大学求是特聘教授陈昆松教授担任主编，浙江大学生工食品学院叶兴乾教授、欧洲科学院院士Mondher Bouzayen教授任共同主编。

主要殊荣：中科协科技期刊国际影响力提升计划入选期刊（2019年后为“期刊卓越行动计划”）、《食品科学与工程领域高质量科技期刊分级目录》T1期刊、中国农林核心期刊（A类期刊目录），并多次入选“中国最具国际影响力/国际影响力优秀学术期刊”。

数据库收录情况：SCI、Scopus、FSTA、中国科学引文数据库CSCD（核心库）等。

审稿流程：1-3天完成初审，1个月内完成同行评审，录用后快速上网（牛津学术）并可全文免费阅读下载（OA）。

期刊网站：https://academic.oup.com/fqs

投稿网址：https://mc.manuscriptcentral.com/fqs

最新影响因子：4.4；5年影响因子：4.8

最新分区：农林科学大类一区

主要刊文范围：食品品质、食品安全、食品检测、食品营养健康、食品加工制造、食品原料、食品生物化学、食品微生物学、食品风味与感官、农产品产后处理与保鲜、食品包装，以及其他食品品质与安全相关的主题（详见：https://academic.oup.com/fqs/pages/About）

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  1. CONNECT:[ UseTime:0.000599s ] mysql:host=127.0.0.1;port=3306;dbname=i460;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `fenlei` [ RunTime:0.000954s ]
  3. SELECT * FROM `fenlei` WHERE `fid` = 0 [ RunTime:0.000329s ]
  4. SELECT * FROM `fenlei` WHERE `fid` = 63 [ RunTime:0.000326s ]
  5. SHOW FULL COLUMNS FROM `set` [ RunTime:0.000667s ]
  6. SELECT * FROM `set` [ RunTime:0.000241s ]
  7. SHOW FULL COLUMNS FROM `article` [ RunTime:0.000762s ]
  8. SELECT * FROM `article` WHERE `id` = 550499 LIMIT 1 [ RunTime:0.000515s ]
  9. UPDATE `article` SET `lasttime` = 1781286407 WHERE `id` = 550499 [ RunTime:0.002775s ]
  10. SELECT * FROM `fenlei` WHERE `id` = 64 LIMIT 1 [ RunTime:0.000309s ]
  11. SELECT * FROM `article` WHERE `id` < 550499 ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000532s ]
  12. SELECT * FROM `article` WHERE `id` > 550499 ORDER BY `id` ASC LIMIT 1 [ RunTime:0.000447s ]
  13. SELECT * FROM `article` WHERE `id` < 550499 ORDER BY `id` DESC LIMIT 10 [ RunTime:0.003335s ]
  14. SELECT * FROM `article` WHERE `id` < 550499 ORDER BY `id` DESC LIMIT 10,10 [ RunTime:0.006468s ]
  15. SELECT * FROM `article` WHERE `id` < 550499 ORDER BY `id` DESC LIMIT 20,10 [ RunTime:0.001207s ]
0.104422s