郑州大学姚明浩等 | 活性氧清除水凝胶促进糖尿病小鼠伤口愈合

本研究论文聚焦活性氧清除水凝胶促进糖尿病小鼠伤口愈合。活性氧 (ROS) 的过量产生和细菌感染是糖尿病足溃疡伤口愈合的棘手障碍。在此，我们设计了一种新方法，利用多功能水凝胶实现自级联的葡萄糖消耗和ROS清除，从而改变糖尿病伤口微环境。本研究采用聚乙烯醇 (PVA)、硼砂 (Borax)、低聚原花青素 (OPC) 和纳米酶 (AuPt@PDA) 一步法制备了PVA/Borax/OPC/AuPt@PDA (PBON) 水凝胶。 PBON水凝胶与近红外治疗相结合，可通过葡萄糖消耗、清除ROS、光热治疗 (光热转换率 81.9%)、可变形性等作用，与糖尿病高运动区复杂多变的微环境相匹配，从而通过血管生成和胶原沉积促进表皮再生，促进糖尿病小鼠髋关节附近伤口的愈合。这种方法为运动区域的糖尿病伤口提供了一种简单、安全、高效的治疗方法。

引用本文（点击最下方阅读原文可下载PDF）

Liu K, Jin R, Yang M, et al., 2025. Reactive oxygen species scavenging hydrogel for wound healing in diabetic mice. Bio-des Manuf 8(5):742–758. https://doi.org/10.1631/bdm.2400375

图1 负载AuPt@PDA纳米酶的多功能水凝胶用于促进糖尿病小鼠移动区域的伤口愈合。(a) 多功能PBON水凝胶及PBON的合成示意图，实现自级联式葡萄糖消耗与活性氧清除；(b) 通过简单混合快速制备的PBON水凝胶敷料，通过止血、降糖、抗炎、抑菌及血管再生作用促进糖尿病创面愈合

图2 AuPt@PDA纳米颗粒表征及PBON水凝胶的SEM与EDS图像。(a) PDA纳米颗粒与AuPt@PDA纳米颗粒的TEM图像；(b) PBO和PBON₂水凝胶照片（P代表聚乙烯醇，B代表硼砂，O代表OPC，N代表AuPt@PDA纳米颗粒）；(c) 水凝胶SEM图像及孔径分布图；(d) PBON₂水凝胶的EDS谱图

图3 PBON水凝胶的组织粘附、拉伸和形状适应性、自愈合、可注射、可去除以及易剥离性。(a) 搭接剪切测试示意图；(b) 水凝胶对猪皮的粘附强度；(c) PBON₂水凝胶的拉伸性能照片；(d) 不同PBON水凝胶的拉伸应力-应变曲线；(e) 不同PBON水凝胶的弹性模量；(f) PBON₂水凝胶对模具的形状适应性；(g) PBON₂水凝胶对猪皮创面的形状适应性；(h) PBO和PBON₂水凝胶的自愈合性；(i) PBON₂水凝胶的可注射性；(j) PBON₂水凝胶的可去除性；(k) PBON₂水凝胶的易剥离性

图4 PBON水凝胶的GOx活性、CAT活性及抗氧化能力。 (a) 水凝胶的GOx活性；(b) 水凝胶的产氧量；(c) DCF荧光强度；(d) 水凝胶对H₂O₂的清除率；(e) 水凝胶对DPPH自由基的清除率；(f) 水凝胶对羟基自由基的清除率；(g) 水凝胶的SOD活性；(h) 新鲜培养基处理后的L929细胞的DCF 荧光染色图像（对照组），500 µmol/L H₂O₂处理组（H₂O₂组），500 µmol/L H₂O₂ + 20 µL PBO水凝胶处理组（PBO 组），以及 500 µmol/L H₂O₂ + 20 µL PBON₂水凝胶处理组（PBON₂组）

图5 金黄色葡萄球菌感染的糖尿病伤口模型的建立与治疗。(a) STZ诱导糖尿病小鼠模型、感染伤口的建立及水凝胶治疗示意图；(b) 血糖监测与体重变化曲线；(c) PBON₂水凝胶在近红外照射与非照射状态下的小鼠创面热成像图；(d) 伤口愈合过程的第0、3、7、14、21天照片及愈合进程追踪图；(e) 对照组、PBO组、PBON₂组及PBON₂+NIR组伤口愈合率的三维直方图

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